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The healthcare professional cannot assume that, simply because items
have gone through a sterilization process, they are sterile or that the conditions necessary
for sterilization were present. For this reason, a sound sterility assurance program
utilizing regular use of both chemical and biological monitors must be in place.
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However, in spite of a comprehensive equipment maintenance program,
there may be an occasional sterilization failure. (NOTE: Please see the Education Profile,
"Causes of Sterilization Failure.") Following are general guidelines to follow in case of such
an event:
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Make sure that your sterilizer is being operated using the correct times, temperatures, and pressures. (Consult sterilizer operator's manual.)
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Do not overload the sterilizer chamber. (Check sterilizer operator's manual for recommended load size.)
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Select packaging appropriate for your sterilizer.
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If using a tabletop sterilizer, avoid double wrapping, as the sterilant may not be able to penetrate to the center of the pack.
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Do not interrupt the sterilization cycle.
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Do not stack instruments. Place unwrapped instruments in a single layer to allow the sterilant (steam, chemical vapor, dry heat, etc.), to contact all surfaces. Place packs or packages on edge to facilitate sterilant penetration of packaging materials.
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Perform routine cleaning of unit and gasket according to manufacturer's directions.
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Retest the sterilizer, following these suggested guidelines.
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If your results continue to be positive, discontinue use of the unit until an authorized dealer, or a reputable sterilizer repair service services it.
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